Journal: Journal of Biological Chemistry

Article Title: Molecular Cloning and Expression of a Novel Chondroitin 6-O-Sulfotransferase

doi: 10.1074/jbc.m002101200

Figure Lengend Snippet: FIG. 1. Nucleotide and deduced amino acid sequences of the novel sulfotransferase cDNA. The putative membrane spanning do- main is underlined, and four potential N-glycosylation sites are marked by asterisks. The presumptive polyadenylation signal ATTAAA is boxed. The sequences are numbered relative to the translation initia- tion site, which begins at the first in-frame ATG codon. The location of an intron is indicated by an arrowhead.

Article Snippet: An additional sequence was obtained by 59-rapid amplification of the cDNA ends (RACE) with the human brain Marathon-ReadyTM cDNA kit (CLONTECH, Palo Alto, CA) using an antisense primer, 59-CTTGGGAACCGAGGAGACTGGTTC-39 (the nucleotide sequence corresponding to 237–260; see Fig. 1) and an anchor primer provided by the company.

Techniques: Membrane, Glycoproteomics

Journal: Genes and immunity

Article Title: Cloning of murine IL-22 receptor alpha 2 and comparison with its human counterpart.

doi: 10.1038/sj.gene.6364104

Figure Lengend Snippet: Figure 1 Coding sequence of murine IL-22Ra2 cDNA. (a) Agarose gel electrophoresis of coding murine IL-22Ra2 cDNA from lymph nodes from three BALB/c mice amplified by PCR. L, ladder; NTC, non-template control. (b) Comparison of coding cDNA sequences of human IL- 22Ra2 variant 1 and mouse IL-22Ra2. Nucleotide–nucleotide alignment shows an overall homology of 69%. The human sequence derived from exon 4 is underlined. Exon boundaries are marked by arrows. Within different individuals and tissues of BALB/c mice, varying nucleotides are marked in bold (frequent nucleotides are indicated within the sequence, rare nucleotides are indicated below). The nucleotide at position 447 that differs from previously described data16 is indicated in italic. h, human; m, mouse.

Article Snippet: A comparative genomic alignment of mouse and human IL-22Ra2encoding gene sequences was performed using the PipMaker program (http://bio.cse.psu.edu/pipmaker).18 Prediction of mouse IL-22Ra2 signal peptide length was carried out using Signalp version 2.0.19 Identification of full-length sequence and amplification of the coding region of murine IL-22Ra2 mRNA The full-length murine IL-22Ra2 mRNA sequence was obtained by 50 and 30 RACE-PCR using the mouse spleen Marathon-Readyt cDNA kit in combination with the Advantages-HF 2 PCR kit (both from BD Clontech, Heidelberg, Germany), and the gene-specific primers 50- ATGATGCCTAAGCATTGCCTTC-30 and 50-TCAGACCTTCAATTTCAACAGCTC-30.

Techniques: Sequencing, Agarose Gel Electrophoresis, Amplification, Control, Comparison, Variant Assay, Derivative Assay

Journal: Genes and immunity

Article Title: Cloning of murine IL-22 receptor alpha 2 and comparison with its human counterpart.

doi: 10.1038/sj.gene.6364104

Figure Lengend Snippet: Figure 3 Comparison of predicted amino-acid sequences of murine IL-22Ra2 and human IL-22Ra2 variant 1. Between mouse and human, identical (|) and similar (:) amino-acid positions are illustrated. Mouse and human sequences are 58 and 66% identical and similar, respectively. The 32 aa corresponding to human exon IV are underlined. The ends of predicted sequences of signal peptides are indicated by arrows. For the mouse protein, two alternative signal peptides were calculated (black arrow: most probable prediction; gray arrow: prediction with lower but also significant probability). Potential N-glycosylation sites (N-X-S, N-X-T) are boxed. Conserved amino-acid positions (identical denoted by black boxed letters, similar by gray boxed letters) characteristic for the CRF2 are shown. Amino-acid variations in the mouse protein corresponding to variations in the cDNA sequence shown in Figure 1 are marked in bold or italic.

Article Snippet: A comparative genomic alignment of mouse and human IL-22Ra2encoding gene sequences was performed using the PipMaker program (http://bio.cse.psu.edu/pipmaker).18 Prediction of mouse IL-22Ra2 signal peptide length was carried out using Signalp version 2.0.19 Identification of full-length sequence and amplification of the coding region of murine IL-22Ra2 mRNA The full-length murine IL-22Ra2 mRNA sequence was obtained by 50 and 30 RACE-PCR using the mouse spleen Marathon-Readyt cDNA kit in combination with the Advantages-HF 2 PCR kit (both from BD Clontech, Heidelberg, Germany), and the gene-specific primers 50- ATGATGCCTAAGCATTGCCTTC-30 and 50-TCAGACCTTCAATTTCAACAGCTC-30.

Techniques: Comparison, Variant Assay, Glycoproteomics, Sequencing